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BBloedCheckupLab made simple—
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BBloedCheckupLab made simple
  • Blood tests
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    View all blood tests
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    • Contact us
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BBloedCheckupLab made simple
  • Blood tests
    • Test packages
    • Hormones
    • Cardiovascular
    • Thyroid
    • Vitamins
    • Allergies and intolerances
    • Custom test request
    View all blood tests
  • At-home tests
    • Test packages
    • STDs
    • Hormones
    • Heart
    • Thyroid
    • Vitamins
    • Drug use
    View all at-home tests
  • How it works
    • Blood collection at a location
    • At-home tests
      • Fingerstick
      • Urine
      • Saliva
      • Stool
      • Hair
      • Swab
    • Locations
    • Laboratories
    • Tests A-Z
    • Fasting guidance
    • Shipping
    • Risks
    • Incidental findings
  • Contact
    • Contact us
    • Custom testing
    • FAQ
    • Client reviews
    • For business
Blood draw

Supplemental: Testosterone Replacement Therapy (TRT)

Complete hormonal analysis with testosterone, DHEA-S, thyroid, blood count, liver function, kidney function, lipids, glucose, HbA1c, insulin, CRP, vitamin D and ferritin.

€ 335,00

Blood collection at collection point

Schedule your blood draw yourself

  1. 01

    Order your test

    After your order you receive instructions by email

  2. 02

    Schedule your appointment

    Make an appointment at a collection point near you

  3. 03

    Visit the collection point

    Blood collection at 1,000+ locations across the Netherlands

  4. 04

    Receive your result

    Digitally by email once the analysis is complete.

See how it works

Blood collection at collection point

How it works

See how it works
  1. 01

    Order your test

    After your order you receive instructions by email

  2. 02

    Schedule your appointment

    Make an appointment at a collection point near you

  3. 03

    Visit the collection point

    Blood collection at 1,000+ locations across the Netherlands

  4. 04

    Receive your result

    Digitally by email once the analysis is complete.

See how it works

What is Supplemental TRT Testing?

The Supplemental TRT blood test is the most comprehensive and advanced TRT monitoring available. This test adds advanced hormonal markers, thyroid function, complete blood count with differentiation, inflammatory markers, and additional cardiovascular risk factors. It is the ultimate test for men who want maximum insight into their hormonal health during TRT, in complex cases, or when standard TRT monitoring does not adequately explain symptoms. This test is recommended for persistent symptoms despite TRT, suspected coexisting conditions, or for men seeking optimal health.

What is tested?

  • Testosterone (total) – Primary male hormone
  • Free testosterone – Biologically active form
  • SHBG – Sex Hormone Binding Globulin
  • LH – Luteinizing hormone
  • FSH – Follicle-stimulating hormone
  • Estradiol – Main estrogen
  • Prolactin – Pituitary hormone
  • DHEA-S – Dehydroepiandrosterone sulfate
  • TSH – Thyroid function
  • fT4 – Free thyroxine
  • fT3 – Free triiodothyronine
  • Complete blood count with differentiation – Hemoglobin, hematocrit, leukocytes, platelets + subtypes
  • Complete liver function – ALT, AST, Gamma-GT, Alkaline phosphatase, Bilirubin
  • Kidney function – Creatinine, eGFR, urea
  • Extended lipid profile – Cholesterol, HDL, LDL, Triglycerides, Non-HDL, Apo B
  • Glucose and HbA1c – Blood sugar regulation
  • CRP – Inflammatory marker
  • Vitamin D – For bone health and immune system
  • Ferritin – Iron storage
  • Insulin (fasting) – Metabolic health

Why should you get Supplemental TRT testing?

This most comprehensive TRT test is suitable when standard TRT monitoring is insufficient, for persistent symptoms despite optimal testosterone levels, in complex hormonal problems, or for optimal health monitoring. DHEA-S provides insight into adrenal function. Complete thyroid function (TSH, fT4, fT3) is crucial because thyroid problems can limit TRT effectiveness. HbA1c and insulin monitor metabolic health because TRT can affect insulin sensitivity. CRP measures systemic inflammation. Vitamin D and ferritin are important for optimal TRT response. This comprehensive package is recommended at the start of complex TRT trajectories, for unexplained symptoms, or for men who want to preventively optimize all aspects of their health.

What does it indicate?

The results provide the most complete picture of your hormonal and metabolic health during TRT. In addition to all standard TRT markers, adrenal function (DHEA-S), thyroid function (complete analysis), metabolic health (glucose, HbA1c, insulin), inflammatory status (CRP), nutritional status (vitamin D, ferritin), and advanced cardiovascular markers (Apo B, non-HDL) are also measured. This helps explain why some men respond optimally to TRT while others still experience symptoms despite good testosterone levels. Often, coexisting conditions such as hypothyroidism, adrenal fatigue, insulin resistance, or vitamin deficiencies are the explanation.

What does a high level mean?

Elevated testosterone requires dose reduction. Very high hematocrit (above 54%) is dangerous and requires immediate intervention. Elevated estradiol can be treated with aromatase inhibitors. High DHEA-S can indicate adrenal conditions. Elevated thyroid function (low TSH, high fT4/fT3) can worsen TRT side effects. Elevated HbA1c and insulin indicate insulin resistance or diabetes. High CRP indicates systemic inflammation. High Apo B indicates increased cardiovascular risk despite normal LDL. These comprehensive markers help optimize all aspects of health.

What does a low level mean?

Low testosterone during TRT requires dose increase. Low DHEA-S can indicate adrenal fatigue which limits TRT effectiveness and may require supplementation. Low thyroid function (high TSH, low fT4/fT3) explains persistent fatigue despite optimal testosterone levels and requires thyroid treatment. Low vitamin D limits TRT response and bone health. Low ferritin can explain fatigue. Low HDL and high Apo B increase cardiovascular risk. This complete analysis helps identify and optimize all factors for maximum TRT effectiveness and overall health. It is the gold standard for complex TRT monitoring and preventive healthcare.

Preparation and sampling

Fasting: no food for 12 hours before collection

  • Do not eat anything for 8 hours before the sample is taken and drink only water. Ideally have your blood taken in the morning before breakfast.Applies to: Glucose
  • Do not eat anything for 12 hours before the sample is taken and drink only water. After a meal the value is many times higher.Applies to: Insuline
  • Fasting is not required, but it gives the most accurate result: in that case do not eat for 12 hours before the sample is taken and drink only water. If the result is very high, have the test repeated while fasting.Applies to: Triglyceriden
  • Preferably do not eat for 12 hours before the sample is taken and drink only water. Also avoid an extra fatty meal the evening before: in some people the value is still higher the next morning after a fatty meal.Applies to: Alkalische fosfatase
  • Preferably do not eat for 8 hours before the sample is taken and drink only water. Testosterone is temporarily lower after a meal.Applies to: Testosteron
  • Have your blood taken in the morning before 11.00, because testosterone drops during the day. If you work nights, do not have your blood taken straight after a night shift.Applies to: Testosteron
  • Do you have a menstrual cycle? Then have your blood taken on day 2 to 5 of your cycle (day 1 is the first day of your period). Without a cycle, on the pill or after menopause the day does not matter.Applies to: LH, FSH, and Oestradiol
  • Have your blood taken in the morning, at least 1 hour after getting up.Applies to: Prolactine
  • Do not drink alcohol the day before the sample is taken, because alcohol raises triglycerides.Applies to: Triglyceriden
  • Do you take biotin (vitamin B8, often in hair and nail supplements)? Stop at least 2 days before the sample is taken, or 4 days for a high dose.Applies to: Insuline, TSH, Vrij T4, Vrij T3, Testosteron, SHBG, LH, FSH, Oestradiol, Prolactine, DHEA-S, and Vitamine D (25-OH)
  • Take your thyroid medication after the blood test.Applies to: Vrij T4
  • Take it easy: stress and heavy exercise shortly before the sample is taken raise prolactin.Applies to: Prolactine
More about fasting and preparation

Risks and downsides of this test

  • Blood draw at a collection point. A needle in a vein is a safe routine procedure. A bruise or slight dizziness happens; an infection of the puncture site is rare.
  • No test is one hundred percent reliable. A result can point to something that is not there, or miss something that is. A result is a snapshot and does not make a diagnosis.
  • An abnormal result has consequences. It can lead to follow-up tests with your GP, and to worry that turns out to be unnecessary. Follow-up tests or treatment can have downsides of their own.
  • You may find something other than what you were looking for. For this test that could be a blood disorder (such as leukaemia) or carrying an inherited blood disorder (such as thalassaemia). This happens occasionally. The test is not intended to find these. During checkout you choose whether you want to be informed about such an incidental finding.
All risks and downsidesOur policy on incidental findings

Blood count and blood group13

Iron1

Heart and vessels6

Sugar and metabolism3

Liver and pancreas5

Kidneys and electrolytes3

Thyroid3

Hormones8

Vitamins, minerals and amino acids1

Inflammation and immunity1

Always discuss an abnormal result with your GP. If a result is strongly abnormal, we try to reach you as soon as possible. Read your result yourself as well and contact your GP if in doubt. A test does not make a diagnosis. Read more about risks and results

Independent information sources

We recommend consulting these independent sources before ordering a preventive health check.

  • Client brochure PGO (NVvPG, September 2023)
  • RIVM: Health tests
  • Thuisarts.nl
  • Allesovertesten.nl (NVKC)

Practical information

Frequently asked questions

How does it work from order to result?

After ordering we generate a referral letter and email you the instructions. You pick a collection point yourself and book an appointment there. Blood is drawn at the location, processed at the lab, and your result arrives digitally.

How quickly can I get tested?

Often within a few working days. How soon you can be seen depends on how busy the location is and the type of analysis. For specialist analyses we first ship collection materials, which takes a bit longer.

Do I need to book an appointment?

At most collection points yes. Some accept walk-ins. This is clearly indicated per location. You choose the location that works best for you.

Do I need to fast before the test?

That depends on the test. Whether fasting is required, and from what time, is clearly stated in the instructions you receive after ordering. No surprises at the counter.

When and how do I receive my result?

Usually within a few working days after collection. Turnaround time varies by analysis; analyses sent to an external lab can take longer. You receive your laboratory report securely by email. It lists the measured values, units, reference ranges and any flags added by the laboratory.

Where can I go for collection?

We work with 1,000+ collection points across the Netherlands. After ordering you'll see which locations near you are available and you pick where you'd like to go.

View all frequently asked questions

Personal help

Contact us

Not sure which test is right for you? We’re happy to help.

WhatsApp+31 (0)412 23 69 61E-mailinfo@bloedcheckup.nlCollection points1,000+ across the Netherlands

Weekdays 09:00 – 17:00

Didn't find what you're looking for?Request a custom test

Verified reviews

View allView all reviews

Client experiences

90% recommend us434 verified reviews

“Very positive experience”

From ordering to having my blood drawn, everything went smoothly and was well organised. I really appreciated being able to visit a blood collection location in my own town so quickly. I received clear instructions beforehand, and the results arrived promptly by email too. Very satisfied!

ElineVerified

“Good communication”

This company responds to questions thoroughly and promptly. The entire process, from requesting the test to having blood drawn and receiving the results, runs very smoothly. Overall, I am very satisfied!

Jacques QuipèreVerified

“Wide choice of tests, fast and affordable”

You can choose the tests yourself. Fast delivery and processing, clear instructions and affordable.

SMVerified

“I am satisfied with the service”

I received a referral by email, just like from a GP. I did not have to send anything by post and could quickly visit a local blood collection centre. The results were fast and clear. The price was good, not too expensive, and all messages were secure.

kraljicaVerified

“A very good experience”

A very good experience! I received quick answers to my questions, a custom blood test was put together, everything was arranged smoothly, and I could have my blood drawn by a healthcare professional nearby. The results were available within a few days. In short, very satisfied!

PhilVerified

“Very good customer service”

The customer service is very good. I had a complex request, which caused an issue at the blood collection centre. Customer service resolved it very well. They also offer a wide range of tests at a reasonable price.

Marc EekhoutVerified

“Everything was well organised”

Everything was well organised. The products were delivered within a few days and, after sending in the samples, the results arrived within a few days too. Communication and information throughout the process were clear and friendly.

martijnVerified

“Fantastic service”

Fantastic service! Very good customer service that responds quickly and thinks along with you. Good prices. I will definitely use BloedCheckup's services again.

kambalesVerified

“Very happy to have found this business”

Very happy to have found this business. We need to check our blood more often :) The process was easy. They sent me the kit at home, then I had to go to a clinic with the papers and they knew what they had to do. I got the results in less than a week.

DenisaVerified

“Smooth process and good support”

Amazing website experience. I ordered various tests for 2 people. We went to one of the supported centers and took samples. Also after the results were ready, I needed a letter from the laboratory and they prepared the letter for me in no time. Definitely recommended.

Majid HajiheidariVerified

“Amazing service!”

Received a packet with everything needed, went to a walk in clinic (Amsterdam DC Clinic) where my blood was drawn and I was out the door within 5 minutes, and then after posting the samples, received results 2 days later. Results were easy to understand as well. Amazing service, would highly recommend!

Trudi CoetzeeVerified

“Great customer service”

I ordered a test through bloedcheckup.nl but later realized I no longer needed it. I contacted their support team, and they were very quick, responsive, and helpful in handling my cancellation. The whole process was smooth and efficient. Great customer service!

Elif Göksu BecerikVerified
BBloedCheckupLab made simple

Blood tests that actually tell you something — working with ISO-certified laboratories. Fast results, no GP referral required.

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